How would you best describe your practice?

• Academic
• Community
• Commercial
• Other

Rank the factors that influence your choice of DNA sequencing technologies in your practice (where 1 is the most influential and 5 is least influential) Please rank all.

• Accuracy
• Cost
• Ease of use
• Read length
• Throughput

Do you use short-read sequencing technologies?

• Yes
• No

What is the percentage breakdown of short-read sequencing technologies you currently use? /*Displayed if use short-read technology = yes*/

• Illumina (e.g.,NovaSeq, HiSeq, MiSeq, etc.): 
• Thermo Fisher Scientific (e.g., Ion Torrent, Ion GeneStudio, etc.): 
• Oxford Nanopore Technologies (e.g., Flongle, Rapid Barcoding Kit): 
• Other:

What is the percentage breakdown of short-read sequencing technologies you expect to use in 1 year? /*Displayed if use short-read technology = yes*/

• Illumina (e.g.,NovaSeq, HiSeq, MiSeq, etc.):
• Thermo Fisher Scientific (e.g., Ion Torrent, Ion GeneStudio, etc.):
• Oxford Nanopore Technologies (e.g., Flongle, Rapid Barcoding Kit):
• Other:

Please elaborate if you indicated "Other" above. If "Other" is 0, please indicate "NA". /*Displayed if use short-read technology = yes*/

What are pros and cons of Illumina's short-read technologies? Please be specific. /*Displayed if use short-read technology = yes*/

What are pros and cons of Thermo Fisher Scientific's short-read technologies? Please be specific. /*Displayed if use short-read technology = yes*/

What are pros and cons of Oxford Nanopore's short-read technologies? Please be specific. /*Displayed if use short-read technology = yes*/

Do you currently use long-read sequencing technologies?

• Yes
• No

What is the percentage breakdown of long-read sequencing technologies you currently use? /*Displayed if use long-read technology = yes*/

• PacBio (e.g., Sequel lle, RS II, etc.): 
• Oxford Nanopore Technologies (e.g., MinION, GridION, PromethION, etc.): 
• Bionano Genomics (e.g., Saphyr system): 
• Other:

What is the percentage breakdown of long-read sequencing technologies you expect to use in 1 year? /*Displayed if use long-read technology = yes*/

• PacBio (e.g., Sequel lle, RS II, etc.):
• Oxford Nanopore Technologies (e.g., MinION, GridION, PromethION, etc.):
• Bionano Genomics (e.g., Saphyr system):
• Other:

Please elaborate if you indicated "Other" above. If "Other" is 0, please indicate "NA".  /*Displayed if use long-read technology = yes*/

What are pros and cons of PacBio's long-read technologies? Please be specific.  /*Displayed if use long-read technology = yes*/

What are pros and cons of Oxford Nanopore's long-read technologies? Please be specific.  /*Displayed if use long-read technology = yes*/

What are pros and cons of Bionano Genomics' long-read technologies? Please be specific.  /*Displayed if use long-read technology = yes*/

Do you currently use hybrid sequencing technologies?

• Yes
• No

Why or why not?

What is the most promising DNA sequencing technology in your opinion? Please be specific.

What barriers, if any, have you noticed in adopting DNA sequencing technologies in your practice?